Protein Sciences - Roles & Responsibilites
- Design & execute end-to-end protein purification workflows (ab initio or SOP-based) for drug discovery projects from expression to crystallization-ready samples
- Optimize recombinant protein expression across E. coli, yeast, insect (Sf9), and mammalian (HEK/CHO) systems using affinity, IEX, SEC, and HIC chromatography (AKTA/FPLC)
- Support membrane protein purification (detergent screening, nanodiscs, solubilization) and prepare high-quality samples for crystallization/cryo-EM
- Perform QC analytics: SDS-PAGE, Western blot, SEC-HPLC, DLS, CD, endotoxin testing to ensure protein fit-for-purpose for screening or structural studies
- Analyze and interpret assay data using GraphPad Prism, UNICORN, or Empower; present findings to drive molecule progression decisions
- Maintain ALCOA+ compliant eLN documentation, adhere to GLP/GMP SOPs, and ensure 100% lab safety & quality compliance
- Collaborate with structural biology, biophysics, and assay teams; contribute to process improvement, knowledge sharing, and platform innovation
Requirements: M.Sc/M.Tech in Biotechnology/Biochemistry, 9-14 years CRO/biopharma experience, hands-on AKTA/chromatography expertise, SnapGene proficiency
