5-7 years of hands-on experience in cell-based assay development and troubleshooting skills; ability to handle different plate formats for small molecule screening
Reporting to senior team leader, work with a group of in vitro assay biologists, learning and coaching group members to improve skills and productivity
Independently design, conduct, optimize and proactively troubleshoot assays
Develop, optimize and validate biochemical and cell-based assay platforms for the hit identification, lead optimization and functional characterization of small molecules
Work on the bench implementing state-of-the-art technologies for the different assay platforms
Ensure robust and high-quality assays with efficient use of automation and instrumentation
Data processing, interpretation and presentation to cross functional team members
Proposing, planning, quoting, tracking reagent needs, usage and executing timelines for each study
Work closely with other biology groups involved in screening, cell biology, protein biology and ADME, offering integrated drug discovery support alongside chemistry for multiple client-based projects
Job requirements
Extensive work experience in cell biology and biochemistry techniques
Good working knowledge of transcription-based studies, targeted protein degradation, target identification and validation
Cell culture experience and in handling different immortalized cell lines, primary cultures, 3D cultures
Ability to perform transient transfections and generate stable cell lines
Good working knowledge of immunoprecipitation and Western blotting for protein analysis, quantification and in studying protein-protein interactions
Experience in fluorescence microscopy and high-content imagining is an advantage
Experience in targeted protein degradation, target engagement assays (e.g. nanoBRET, CETSA) is an advantage
Experience in CRISPR-CAS9 knock-out and siRNA/shRNA knock-down studies
Experience with site-directed mutagenesis is beneficial but not a must
Candidates must be capable of excellent teamwork in a fast-paced, multidisciplinary environment, where meeting timelines will be essential.
Excellent data analysis and interpretation skills
An ability to overcome technical challenges and exercise sound judgment under pressure
High self-motivation to initiate projects and work independently under aggressive deadlines
A strong technical knowledge with exceptional attention to detail
An excellent personal rapport and an ability to engender trust and respect
An ability to work well as part of a small focussed team and build relationships
An exceptional communication skill and an ease in interaction with all stakeholders
An infectious enthusiasm for life, work and making a difference to the world in which we live
A bright, friendly, flexible personality with agility to switch between different skill sets
An exceptional organisational skill that thrives working in a fast-paced and vibrant team environment
Positivity, focus and a winning mentality allied with kindness
Curiosity, energy, creativity, and bravery with a strong desire to help to build the o2h culture
