About the Role
We are seeking a self-driven Scientist to lead and deliver end-to-end molecular biology projects in our NBE Services portfolio. You will design and execute strategies for engineered antibodies, enzymes, structural proteins, and mutant strains supporting global drug discovery programs in a fast-paced CRO environment.
Key Responsibilities
- Design and execute molecular biology workflows for production of engineered antibodies, enzymes, structural/reagent proteins, and mutant strains
- Lead gene synthesis, cloning, vector design/swapping, antibody gene sequencing, and site-directed mutagenesis projects
- Generate CRISPR-based mutants in prokaryotic systems; perform sequence data analysis and validation
- Execute mRNA isolation, cDNA synthesis, and gene expression analysis using Real-Time PCR
- Support HCP/HCD estimation, data analysis, and protein engineering initiatives
- Oversee plasmid production and chromatographic purification (affinity, IEX, SEC)
- Manage microbial, yeast, and fungal strain engineering, including cell bank preparation and characterization
- Operate and optimize automation/high-throughput platforms (liquid handlers, robotic systems)
- Mentor and lead the molecular biology team; drive project planning, execution, and client communication
- Prepare technical reports, presentations, project proposals, and SOPs with minimal review
- Ensure 100% compliance with GLP/GMP, ALCOA+ data integrity, and lab safety standards
Must-Have Qualifications
- 6-10 years of hands-on industrial or advanced academic experience in molecular biology R&D
- Expertise in:
Cloning (In-Fusion, Gibson, TOPO, ligation-based)
Vector design, gene synthesis, mutagenesis, antibody sequencing
CRISPR-based editing in prokaryotes
RT-qPCR, mRNA/cDNA workflows, sequence analysis
Mammalian/microbial cell culture and strain engineering
SnapGene, Sequencher, Benchling, or equivalent bioinformatics tools
