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Job Description

The Candidate will contribute to our recombinant antibody discovery and developments related activities using antibody phage display technology.

  • Responsible for using antibody phage display platform for discovering antibodies. Reformat recombinant antibodies into other isotypes, species and conformations like bi-specific, and other multi-specific formats.
  • Expression and purification of antibodies in bacterial and mammalian system, their characterization and application specific validation.
  • Efficiently analyse, record and report data; experience with electronic notebook will be an added advantage
  • Prepare presentations, project reports and present in weekly and/or monthly group meetings.
  • Actively participate in non-technical activities required for smooth functioning of the department.

Skills Required

  • Experienced in Basic molecular and protein biology techniques like DNA manipulation and analysis, plasmid extraction, cloning, PCR; Protein Estimation Methods, SDS PAGE, Western Blotting, ELISA etc.
  • Microbial (bacteria / yeast) expression and purification of recombinant proteins
  • Mammalian expression and small scale purification of antibodies and/or recombinant proteins
  • Prior experience in Phage display technology will be given preference.

More Info

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Key Skills

Microbial bacteria yeast expression

DNA manipulation and analysis

Mammalian expression

Plasmid extraction

Protein Estimation Methods

Basic molecular and protein biology techniques

Purification of recombinant proteins

Small scale purification of antibodies

Phage display technology