Perform primary cell culture, including stem cell culture, cell-based functional assays , Transfection through lipid or polymer-based systems, imaging such as immunofluorescence, confocal microscopy and involve in production of cell-derived products.
Develop and optimize protocols for various cell and molecular biology techniques, such as RNA isolation, qRT-PCR, ELISA, SDS-PAGE, and Western blotting. This includes mRNA design principles, invitro transcription and Characterization; Total RNA quantification, protein expression analysis, RNA Biology.
Design and develop non-viral nucleic acid delivery systems, with a focus on platforms for next-generation RNA therapeutics.
Assist in Nanoparticle Characterization techniques like DLS, NTA, TEM and Ribo Green/qPCR-style Encapsulation Assays.
Experience in performing fluorescence/confocal microscopy and quantification of images.
Experience in troubleshooting or optimizing experiments in a fast track mode.
Basic understanding of biostatistical tests to analyze multimodal datasets. Analyze data and prepare reports
Prepare SOPs for various experimental procedures
Prepare data for internal and external presentations, communications and publications
Participate in team meetings and scientific discussions
Required Skills / Experience
PhD with two years of Postdoctoral experience in the fields of Cell Biology , Molecular Biology, Biochemistry, Drug Delivery, Nanobiotechnology
Experience in mammalian cell culture, cell-based bio-assays, PCR/qRT-PCR, ELISA, nucleic acid delivery, nanoparticle synthesis , RNA Biology and characterization are mandatory requirements for this role.
