We are partnering with a rapidly growing biotech company to hire for multiple roles in Bioprocess Downstream (RE/RA/SRA) based in Bengaluru.
Job Title: Research Executive / Research Associate / Senior Research Associate – Bioprocess Downstream
Required Qualifications: 0–8 years of industrial experience in protein/large molecule purification (lab scale to pilot scale).
Education:
- M.Tech in Biotechnology or Chemical Engineering
- M.Sc. Biotechnology
- M.Sc. Biochemistry
- PhD in Life Sciences or related fields
Roles and Responsibilities:
- Carry out downstream processes from the following purification steps.
- Develop the following purification steps for process scale-up:
Purification Steps
- Harvest & Clarification – Centrifugation, depth filtration, microfiltration
- Cell disruption (homogenization, chemical lysis)
- Chromatography-based purification
- Ultrafiltration/Diafiltration (TFF)
Knowledge and Experience:
Practical experience and/or theoretical knowledge in any or all of the following purification steps:
- Harvest & Clarification – Centrifugation, depth filtration, microfiltration
- Knowledge/experience in various types of resins such as Affinity chromatography (His-tag, Protein A/G/L), Ion exchange (CEX/AEX), Hydrophobic interaction chromatography (HIC), Mixed-mode chromatography, Size exclusion chromatography (SEC)
- Ultrafiltration/Diafiltration (TFF)
- Impurity Clearance – Host cell protein (HCP) reduction, DNA and endotoxin removal, Viral inactivation and filtration
- Formulation & Fill – Buffer exchange, concentration, stability optimization
- Operation of purification equipment like chromatography systems, TFF systems, etc.
