plasmid vector construction, BLI, Molecular Biology, immunogen designs, IHC, antibody development, Western Blotting, Glp, Spr, protein-protein interaction, Protein Expression, IF, conjugation chemistries, Elisa, standard operating procedures, next generation sequencing, Protein Purification, biophysical characterization, high-throughput cloning, sds-page, affinity measurements, protein biology, epitope binning, proximity ligation